THE ACTIVITY AND ISOFORMS OF SUPEROXIDE DISMUTASE IN TISUES OF REPRODUCTIVE ORGANS OF RATS

  • N. Kuzmina Institute of Animal Biology of NAAS, 38, Stusа str., Lviv, 79034, Ukraine
  • D. Ostapiv Institute of Animal Biology of NAAS, 38, Stusа str., Lviv, 79034, Ukraine
  • A. Chajkovska State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives, 11, Donetska str., Lviv, 79019, Ukraine
  • A. Panych State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives, 11, Donetska str., Lviv, 79019, Ukraine
Keywords: SUPEROXIDE DISMUTASE, ISOFORMS, REPRODUCTIVE ORGANS, SPERMATOZOA, ELECTROPHORESIS.

Abstract

The aсtivity of superoxide dismutase, the content of its isoforms in testis, epididimis, and in epididimal spermatozoa of pubescent rat males were studied.

The experiments were performed on adult male rats (age 5 - 6 months). After decapitation, the testes and the epididimis were taken, from which the spermatozoa were washed with 0.9 % sodium chloride solution. The total protein and activity of superoxide dismutase (SOD) were determined in the supernatant and the sperm suspension. SOD isoforms were detected after electrophoresis in 10 % polyacrylamide gel by specific staining of gel plates with nitrosine tetrazolium.

The highest activity was found to be in the tissue of the testes (18.2 ± 2.59 IU/mg of protein), less on 34.6% in the epididymis and the lowest on 46.7% in epididymal sperm.

Electrophoresis in 10 % polyacrylamide gel and specific staining in the tissues of testis, epididymis, and epididymal sperm of rats revealed five major SOD isoforms, which, depending on the speed of movement in PAAG, were labeled, from maximum to least mobile, such as S1, S2, S3, S4 and S5. The specifity of testicular tissue is the presence of two minor isoforms (S1a- and S1b-isoforms), which are characterized by high electrophoretic mobility and lower intensity of manifestation, and the color disappears over time, unlike other proteins of the enzyme.

It was found that the SOD isoenzyme spectrum is characterized by tissue specificity and depended on the physiological and functional features of the tissue of the reproductive organs of males and spermatozoa. In the testis tissue, S2 isoform was in the largest amount– 36.7 ± 1.91%, less and almost the same content of S1-, S3- and S4-isoforms (18.0 ± 3.27, 19.3 ± 3.98 and 15.7 ± 2.15%) and the least – S5-, S1a- and S1b-isoform (6.2 ± 1.20%; 2.2 ± 0.03 and 1.9 ± 0.01%). In the epididimis tissue, the content of the S3-isoform was higher on 17.9% and S2- and S1- respectively on 7.9% and 4.9%, respectively, compared to the testis. SOD isoenzymes of epididymal spermatozoa are characterized by a high content of S2 isoforms, which is more on 11.2% than in the testis, and the content of S1 isoforms, by contrast, is lower on 10.0%. The established differences in the content and activity of individual isoforms of the enzyme indicate the existence of tissue specificity of SOD proteins, due to the functional characteristics of the studied tissues. Optimal protection of sperm from O2• - in the process of their differentiation and maturation is ensured by the activity of different SOD isoforms, confirming the established differences in the isoenzyme spectrum.

References

Antonyuk, S.V., Strange, R.W., Mark, S.L., Samar, H.S. (2009). The structure of human extracellular copper–zinc superoxide dismutase at 1.7 Å resolution: insights into heparin and collagen binding. Journal of molecular Biology, 388, 310-326.

Beauchamp, C. & Fridovich, I. (1971). Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels, Anal. Biochem. 44, 276–287.

Bucak, M. N., Ateşşahin, A., Varışl, Ö., Yüce, A., Tekin, N. (2007). The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen: Microscopic and oxidative stress parameters after freeze–thawing process. Theriogenology, 67, 1060-1067.

Chevari, S. N., Andyan, T. A. Shtrenger, Yа. I. (1991). Opredeleniye antioksidantnikh parametrov krovi i ikh diagnosticheskoye znacheniye v pozhilom vozraste. Lab. Delo, 10, 9–13 [in Russian].

Colagar, A.H. & Marzony, E.T. (2009). Ascorbic Acid in human seminal plasma: determination and its relationship to sperm quality. Journal of Clinical Biochemistry and Nutrition, 45(2), 144-149.

Du Plessis, S., Agarwal, A., Halabi, J., Tvrda, E. (2015). Contemporary evidence on the physiological role of reactive oxygen species in human sperm function. Journal of Assisted Reproduction and Genetics, 32, 509-520.

Kuzmina, N.V. & Ostapiv, D. D. (2008). Aktyvnist superoksyddysmutazy i hlutationperoksydazy v riznykh orhanakh i krovi koriv. Biolohiya tvaryn, 12, 423–429 [in Ukrainian].

Kuzmina, N.V. Ostapiv, D.D., Huleyuk, N..L., Humenets’kyy, I. Yе (2010). Vydovi ta indyvidualni osoblyvosti izoenzymiv superoksyddysmutazy spermy. Ukr. biokhim. Zhurn, 82, 85–86 [in Ukrainian].

Kuzmina, N. V., Ostapiv, D. D., Yaremchuk, I. M. (2010). Izoenzymnyy spektr superoksyddysmutazy v protsesi inkubuvannya eyakulyativ buhayiv. Biolohiya tvaryn, 12, 423–429 [in Ukrainian].

Lowry, O. H., Rosebrough, N. J., Fair, A. L., Randall, R. J. (1951). Protein measurement with Folin phenol reagent. J. Biol. Chem, 193(1), 264–275.

Marnett, L. J. Oxyradicals and DNA damage. (2000). Carcinogenesis. 21, 361–370.

Nowicka-Bauer, K. & Nixon, B. (2020). Molecular Changes Induced by Oxidative Stress that Impair Human Sperm Motility. Antioxidants (Basel). 9(2), https://doi.org/10.3390/antiox9020134.

Sakkas, D., & Alvarez, J. G. (2010). Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis. Fertility and Sterilit, 93, 1027-1036.

Thomson, L. K., Fleming, S. D., Aitken, R. J., De Iuliis, G. N., et al. (2009). Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis. Hum. Reprod, 24, 2061–2070.

Published
2020-06-01
How to Cite
Kuzmina, N., Ostapiv, D., Chajkovska, A., & Panych, A. (2020). THE ACTIVITY AND ISOFORMS OF SUPEROXIDE DISMUTASE IN TISUES OF REPRODUCTIVE ORGANS OF RATS. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 21(1), 113-118. https://doi.org/10.36359/scivp.2020-21-1.13