Efficiency biologically active tissue preparation at growing of piglets

  • I. Ya. Kotsyumbas State Scientific-Research Control Institute of Veterіnary Medicinal Products and Feed Additives, Lviv, Ukraine
  • M. I. Zhyla State Scientific-Research Control Institute of Veterіnary Medicinal Products and Feed Additives, Lviv, Ukraine
  • N. E. Lisova State Scientific-Research Control Institute of Veterіnary Medicinal Products and Feed Additives, Lviv, Ukraine
  • A. V. Halkin «ZooVeterinary Center» Ltd, smt. Chkalovske, Chuguevsky district, Kharkiv region, Ukraine
  • O. V. Mychalyuk State Scientific-Research Control Institute of Veterіnary Medicinal Products and Feed Additives, Lviv, Ukraine
  • G. M. Mychalus State Scientific-Research Control Institute of Veterіnary Medicinal Products and Feed Additives, Lviv, Ukraine
Keywords: piglets, haematological, immunological, biochemical indexes, tissue preparation

Abstract

Biologically active preparations, mainly of tissue origin, are widely used in the practice of veterinary medicine. Analysis of literary data shows that the elemental composition of tissue preparations depends on species specificity, therefore the characteristic feature of such means is the ability to provide a wide range of pharmacological action and increase the reactivity of the organism. The preparation of the Splenoton, a solution for injections, manufactured by Zooveterynny Center LLC, contains a complex of biologically active substances (nucleotides, lipids, peptides, vitamins, organic acids and other biologically active substances), which are represented by the lipophilic fraction of the tissues of the spleen of cattle.

The purpose of the study was to investigate the therapeutic efficacy and safety of the Splenoton. The task of the study was to establish the therapeutic effect of the use of the drug for piglets with signs of hypotrophy, as well as to study its effect on the basic morphological, immunological and biochemical parameters of blood of animals. For morphological studies, stabilized EDTA blood was used, and for blood biochemical studies – serum. Morphological parameters of blood of piglets were determined using the automatic analyzer Mythic 18 Vet, biochemical - using a biochemical analyzer (HumaLyzer 3000) using human test kits.

When examining the number of piglets after weaning, the breed "Big White" was selected a group of animals (10 heads) aged 1,5-2 months, in which observed lag in growth, deterioration of appetite, signs of anemia state. From the selected piglets, an experimental group of animals was formed which used the compound of the Splenoton intramuscularly at a dose of 1,0 ml/10 kg body weight 3 times with an interval of 48 hours. The control group of animals was clinically healthy and received an intramuscular isotonic sodium chloride solution of 0.9% at the same dose. Prior to application, and 14 days after using the Splenoton drug, animals in the experimental and control groups were examined for blood samples.

During the period of study in animals, both groups of death and disease were not noted. All indicators of the general clinical status (temperature, pulse, respiration) and laboratory blood tests corresponded to the physiological parameters for this age group. Animals of both groups were active, appetite preserved. The drug was well tolerated by animals, did not cause local and general adverse reactions.

The obtained results of the clinical studies of the preparation of Splenoton, a solution for injection, indicated its effectiveness, which was manifested by improving the overall clinical condition of the piglet`s organism. On the 14th day of the experiment the body weight of animals was increased, processes of hematopoiesis were activated and physiological parameters of the organism stabilized. Preparation Splenoton, in the recommended by the manufacturer dosage, method and multiplicity of the application to young piglets, does not show immunotoxic, hepatotoxic and nephrotoxic effects.

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Published
2019-09-18
How to Cite
Kotsyumbas, I. Y., Zhyla, M. I., Lisova, N. E., Halkin, A. V., Mychalyuk, O. V., & Mychalus, G. M. (2019). Efficiency biologically active tissue preparation at growing of piglets. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 20(2), 67-74. https://doi.org/10.36359/scivp.2019-20-2.09