Theoretical rationalization for quality control of living vaccines by the surface plasmon resonance method
The production of live vaccines requires fast, effective methods to control their quality, including the definition of activity. In modern world, the direction of searching for alternative methods of research that could replace the model of live biological objects is developing. The authors of this article lookin for new alternative ways in the in vitro system to determine the amount of antigen (in this case, the vaccine and native virus of Newcastle disease of chickens) by applying the phenomenon of surface plasmon resonance, that does not require to use of live organisms. For this purpose, a standard strain of the Newcastle virus "La Sota" was used. For conducting studies of the phenomenon of surface plasmon resonance a portable device "Plasmon" with a sensitive sensor element, created on the basis of nanotechnologies in the Institute of Semiconductor Physics V.E. Lashkarev National Academy of Sciences. The virus and serum were used to bind the antigen-antibody in dilutions. A significant difference was found in the kinetic of an interaction of antigen-antibody complex in case of using the production line of virus from freeze-dried vaccine and for the native virus. In addition, better result was shown for vaccine virus in buffer solutions by dilution for at least 1:100 to reduce the effect of impurities in vaccine to obtain a reliable measurement result. Our pilot studies open the new opportunities in the field of vaccine quality control. The applied method does not require to use animals or chicken embryos, which is beneficial both from the point of view of observance of bioethical principles in experiment, and also is less financially expedient. In addition, getting the result is significantly shorter in time in comparison with other methods. Initiated research requires more extensive experiments.
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