The intensity of the oxidation processes in the sperm of the boar at the add of metal nanosuccinates to the ecosperm medium

  • S. Kornjat Institute of Animal Biology NAAS, Lviv, Ukraine
  • I. Yaremchuk Institute of Animal Biology NAAS, Lviv, Ukraine
  • O. Andrushko Institute of Animal Biology NAAS, Lviv, Ukraine
  • D. Ostapiv Institute of Animal Biology NAAS, Lviv, Ukraine
  • M. Sharan Institute of Animal Biology NAAS, Lviv, Ukraine
  • A. Chajkovska State Scientific-Research Control Institute for Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
Keywords: sperm, boar, diluent, survival, nanosuccinate, copper, mangan, zinc

Abstract

The purpose of this study was to investigate the effect of complexes of biologically active substances with nanosites in the form of nanosuccinate of manganese, copper and zinc in the diluent for the dilution of boar sperm "Ecosperm" on the quality of sperm during storage. It has been established that the addition of trace elements nanosuccinates to the diluent ejaculates affects the intensity of the oxidative processes in the semen, the survival and the activity of the enzyme marker fertilizing ability of the sperm succinate dehydrogenase. Respiratory activity of boars' sperm by the addition of trace elements in the form of a nanocompound with succinate changes ambiguously, and depends on the dose in the diluent and the role of the individual trace element in the sperm exchange processes.

The difference between the control and the maximum dose of Zn2 + succinate is 31.5%, and the effect of the increasing content of the complex compound in the sparse sperm on the respiratory activity of medium force (η = 0.410). The effect of the increasing content of Cu2 + - and Mn2 + -succinate in sparse boar sperm on the respiratory activity of medium-strength sperm (respectively, η = 0.628 and 0.539). Using the addition of 0.01 ml /ml succinate Zn2 +(0,6 mg/l), Mn2 + (0,1 mg/l)- and Cu2 +- (0,04 mg/l) in sparse sperm, the highest survival time of boar sperm was obtained compared to the control and the difference was 33.2, 9.2 and 28.4 hours for zinc, manganese and copper respectively.

The optimal concentrations that ensure the normalization of oxidation processes and high sperm survival in diluted boar sperm are: 0.01 ml / ml Mn2+ - (0,1 mg /l), Cu2 + - (0,04 mg/l) and Zn2+ - (0,6 mg/l) nanosuccinate. Increasing the concentrations of trace elements nanosuccinate in the diluent of more optimal values inhibits the respiratory activity of the sperm, and reduces the activity of succinate dehydrogenase.

References

Jablonskyj V.А. Biotehnolohichni i molekularno-henetychni osnovy vidtvorenna tvaryn // V. А. Jablonskyj, S. P. Homyn, V. І. Zaviruha, М. V. Demchuk ta іn. – Lviv: Аfisha, 2009. – 217 s. (in Ukrainian).

Eghbali M. Effects of copper and superoxide dismutase content of seminal plasma on buffalo semen characteristics. / Eghbali M., Alavi-Shoushtari S.M., Rezaii S.A. // Pak. J. Biol. Sci. — 2008. — V. 11. — P. 1964–1968.

Lukjanova L. D. Кislorodzavisimyje processy v кletke i ejo funkcionalnoe sostojanije / L.D. Lukjanova, B. S. Balmuhanov, L. Т. Ugolev. — М.: Nauka, 1982. — 262 s. (in Russian).

Кornyat S. B., Аndrushko О. B., Кuzmina N. V., Bodnar J. V., Sharan М. М., Оstapiv D. D. Іntensyvnist okysno-vidnovnyh procesiv ta vyzyvannja spermijiv knyriv za dodavannja v rozridzeni ejakulaty nanosukcynativ mikroelementiv. Problemy zooinzenerii ta veterynarhoji medycyny, - Zbirnyk naukovyh prac HDZVA,- Vyp.34, ch.2.- s. 70-74.- 2017. (in Ukrainian).

Shtolc К. F. Amperiometricheskoe opredelenie ferrocianida v prisutstvii subkletochnyh struktur / К. F. Shtolc, I. М. Моsolova, L. А. Dronova. Biohimicheskije metody. — М.: Nauka, 1980. — S. 147 – 150. (in Russian).

Chuhrij B. М. Do metodyky vyznachennja aktyvnosti okysluvalnyh fermentiv u spermi buhajiv / B. М. Chuhrij, L. О. Кlevec // Rozvedennja ta shtuchne osimeninnja velykoji rohatoji hudoby. — Кyjiv, 1978. — Vyp. 10. — S. 42–45. (in Ukrainian).

Plohinskij N. А. Rukovodstvo po biometriji dla zootehnicov. – М.: Коlоs, 1969. – 255 s. (in Russian).

Published
2019-09-18
How to Cite
Kornjat, S., Yaremchuk, I., Andrushko, O., Ostapiv, D., Sharan, M., & Chajkovska, A. (2019). The intensity of the oxidation processes in the sperm of the boar at the add of metal nanosuccinates to the ecosperm medium. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 20(2), 352-357. https://doi.org/10.36359/scivp.2019-20-2.46