Physiological and biochemical blood parameters after feeding rats metal complexes with polymer transporter and deficient diet for nutrients

  • R. Branec Institute of Animal Biology of NAAS, Lviv, Ukraine
  • N. Kuzmina Institute of Animal Biology of NAAS, Lviv, Ukraine
  • G. Denys Institute of Animal Biology of NAAS, Lviv, Ukraine
  • Yu. Martyn Institute of Animal Biology of NAAS, Lviv, Ukraine
  • D. Ostapiv Institute of Animal Biology of NAAS, Lviv, Ukraine
  • V. Oleksa Lviv Polytechnic National University, Lviv, Ukraine
  • M. Nagornjak Lviv Polytechnic National University, Lviv, Ukraine
  • I. Dron Lviv Polytechnic National University, Lviv, Ukraine
  • V. Samaryk Lviv Polytechnic National University, Lviv, Ukraine
  • S. Varvarenko Lviv Polytechnic National University, Lviv, Ukraine
Keywords: metals, microelements, polymer transporter, blood, deprivation, rats

Abstract

Physiological and biochemical rat blood parameters after feeding them metal complexes (Fe+2/+3-, Zn+2-, Cu+2 - and Mn+2-) with polymer transporter  (МЕmLPEG400) and deficient diet for nutrients.

When feeding rats deficient diet for nutrients (deprivation) in the organism of animals there is a tendence to activate the processes of erythropoiesis by reducing the concentration of hemoglobin in cells and, accordingly, oxygen absorbing ability of blood. In particular, the number of red blood cells increases in groups: deprivation and addition of Fe+2/+3- and Mn+2-mLPEG400 (р < 0,05), hemoglobin concentration on 6,2, 2,8 and 5,7 % (р < 0,05) when feeding complexes, respectively, Fe+2/+3-, Zn2+- and Mn2+-mLPEG400 and decreases by 5,9 % (р < 0,05) when feeding Cu2+-mLPEG400, and the oxygen-absorbing capacity of the blood is lower (р < 0,001) in deprivation group (control №2; in 4,0 times) and (in 2,5, 3,4, 2,9 and 2,2 times) when feeding complexes, respectively, Cu2+-, Fe2+-, Zn2+- and Mn2+-mLPEG400, compared to values in animals fed a complete diet. The supplemental feeding of MEmlPEG400, the presence of metals and glutamic acid residues, reduce the negative impact of deprivation on hemoglobin concentration and the physiological ability of the blood (erythrocytes) to absorb oxygen.

Addition of complex salts of metals with N-polyoxyethylene derivatives of glutamic acid in the composition of the deficient diet is manifested by a decrease in the activity of GGT, AST and ALT in the blood plasma of rats, which characterizes the weak cyto- and organotoxic effect of added doses of MEmlPEG400 on the cells of liver and kidney.

The nutrient deficiency increases the activity of GPO (p <0.001) in rat blood, and the addition of Cu2+-mLPEG400 increases the activity of GPO and CAT (p <0.05) and the content of TBK-active products (p <0, 01), compared with animals fed a complete diet.

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Published
2019-09-18
How to Cite
Branec, R., Kuzmina, N., Denys, G., Martyn, Y., Ostapiv, D., Oleksa, V., Nagornjak, M., Dron, I., Samaryk, V., & Varvarenko, S. (2019). Physiological and biochemical blood parameters after feeding rats metal complexes with polymer transporter and deficient diet for nutrients. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 20(2), 34-41. https://doi.org/10.36359/scivp.2019-20-2.04