Acute and chronic toxicity of cardiac remedy «Bendamin»
The research of acute and chronic toxicity is a compulsory stage in the study of new remedies, which allows us to assess the hazards of substances for health under short term conditions and determine the class of toxicity and the breadth of therapeutic action. That is why the purpose of the work was to explore the acute and chronic toxicity of cardiac remedy «Bendamin», in particular to determine tolerant, toxic and intermediate-lethal doses for laboratory animals. Experimental studies have been carried out in accordance with the requirements of the medical-biological experiment on the selection of groups of analogues, staging control, observing the same conditions of feeding and retention during the testing and the record of the results. Acute drug toxicity of «Bendamin» was studied in rats and mice for one route of administration – intragastric, which is supposed to be used in veterinary practice. The purpose of determining the chronic toxicity of the drug «Bendamin» is the detection of the harmful effects of the drug in cases of its long-term introduction into the body of experimental animals.
In the research of acute toxicity of cardiac remedy «Bendamin», DL50 failed to determine the value, indicating a low toxicity of the test substance. So DL50 of drug for intragastric administration of white mice is greater than 5000 мg/кg b. w.
The remedy «Bendamin» after administration in the stomach to white mice and rats in the maximum amount does not cause clinical signs of poisoning and deviations in behavior. According to ГОСТ 12.1.007-76, the intragastric administration of «Bendamin», the degree of danger refers to the 4th grade toxicity - low toxicity substances.
During the period of research to determine the chronic toxicity of the drug «Bendamin» probable changes in the behavior of experimental animals were not observed. It should be noted the slight inhibition of the body of the rat experimental group receiving a 10-fold dose of the drug.
In the experimental group of rats, which was administered a 10-fold dose, Inhibition of protein synthesis and detoxification function of the liver was established. There is also a violation of the functional state of the liver, which indicates an increase in the activity of aminotransferases in their blood.
Stekol'nikov, A. A., Vasil'ev, R. M., & Golovacheva, N. V. (2013). Bolezni sobak i koshek. Kompleksnaja diagnostika i terapija. Uchebnoe posobie. M.: SpecLit (in Russian)
Varkholiak, I. S. (2016). Zastosuvannia likarskykh preparativ pry patolohiiakh sertsia i sudyn u sobak ta kishok. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni S. Z. Gzhytskoho. Seriia: Veterynarni nauky, 18, 3(71), 261–265 (in Ukrainian).
Belov, A. D., Danilov, E. P., & Dukur, I. I. (1990). Bolezni sobak: spravochnik. M.: «Agropromizdat» (in Russian).
Martin, M., & Korkorzn, B. (2014). Kardnorespiratornye zabolevanija sobak i koshek. Per. s angl. S.L. Cherjatnikova. M.: «Akvarium Print» (in Russian).
Kozlovskaja, N. G. (2013). Porodnaja predraspolozhennost' sobak i koshek k kardiologicheskim zabolevanijam. Rossijskij veterinarnyj zhurnal, 6, 32–35 (in Russian).
Varkholiak, I. S., & Gutyj, B. V. (2018). Determination of acute toxicity of “Bendamin” drug in laboratory animals. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(92), 209–212. doi: 10.32718/nvlvet9243
Todoriuk, V. B., Hunchak, V. M., Gutyj, B. V., Gufriy, D. F., Hariv, I. I., Khomyk, R. I., & Vasiv, R. O. (2018). Preclinical research of the experimental preparation “Ferosel T”. Ukrainian Journal of Veterinary and Agricultural Sciences, 1(1), 3–9. doi: 10.32718/ujvas1-1.01.
Gutyj, B., Khariv, I., Binkevych, V., Binkevych, O., Levkivska, N., Levkivskyj, D., & Vavrysevich, Y. (2017). Research on acute and chronic toxity of the experimental drug Аmprolinsyl. Regul. Mech. Biosyst., 8(1), 41–45. doi: 10.15421/021708.
Gutyj, B., Paska, M., Levkivska, N., Pelenyo, R., Nazaruk, N., & Guta, Z. (2016). Study of acute and chronic toxicity of ‘injectable mevesel’ investigational drug. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(2), 174–180. doi: 10.15421/201649.
Kotsiumbas, I. Ia., Malyk, O. H., & Patereha, I. P. (2006). Doklinichni doslidzhennia veterynarnykh likarskykh zasobiv. L.: Triada plius (in Ukrainian).
Litvinova, N. V., Filonenko-Patrusheva, M. A., Frantsuzova, S. B., & Khrapak, V. V. (2001). Doklinichni doslidzhennia likarskykh zasobiv: Metodychni rekomendatsii. Pid red. O. V. Stefanova. K.: Avitsenna (in Ukrainian).
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