Chondrogenic differentiation of mesenchal stem cells of the rabbit depending on the type of the cultural system

  • Yu. A. Kharkevych National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
  • R. R. Bokotko National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
  • A. I. Mazurkevych National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
  • O. G. Deryabina SО "Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences", Kyiv, Ukraine
  • V. V. Kovpak National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
  • N. A. Malyuk National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
  • L. V. Kladnytska National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
  • Yu. V. Demyantseva National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
Keywords: mesenchymal stem cells, chondrogenic differentiation, cultural system, monolay, micromass

Abstract

Experimental study concerned with the ability of mesenchymal stem cells to chondrogenic differentiation in vitro depending on the type of culture system with a view to their further clinical use in veterinary medicine to restore the lost functions of damaged articular cartilage is one of the priority directions of scientific research. Reparative ability of cartilage tissue is limited, since the level of physiological and reparative regeneration of cartilage tissue is quite low. Given this, the search continues for such therapeutic agents and techniques that most effectively restore the damaged structure and function of the tissues of the articular cartilage. A special place in this search belongs to biotechnology. The bone marrow mesenchymal stem cells are one of the most studied multipotent populations of adult cells, which, due to their ability to proliferate and differentiate in vitro, is of great importance in clinical veterinary practice in treating animals with articular cartilage lesions. It was established that with the used protocol of chondrogenic differentiation of rabbit bone marrow mesenchymal stem cells in the monolayer most of the cultured cells acquire a filamentous form, which leads to a decrease in the area of ​​their adhesion to the surface of the culture plastic and, as a result, detachment into the culture medium. During cultivation the confluence of cell monolayer in experimental cultures decreased and at 18 days of cultivation was about 5%. During cultivation the mesenchymal stem cells in the form of a precipitate in chondrogenic medium, for the 6th day of the study, it was visually discovered that the precipitated mass of cells at the bottom of the test tube became spherical, indicating cell compactification and are important condition for their differentiation in the chondrogenic direction. This form of micromass was maintained throughout the entire period of cultivation. Comparison of chondrogenic differentiation methods of rabbit bone marrow mesenchymal stem cells in monolayer and micromass showed that the method of differentiation of cells in the micromass is more efficient, since as early as 21 days during the study revealed significantly larger depositions of the extracellular matrix by cells compared to monolayer differentiation.

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Published
2019-09-18
How to Cite
Kharkevych, Y. A., Bokotko, R. R., Mazurkevych, A. I., Deryabina, O. G., Kovpak, V. V., Malyuk, N. A., Kladnytska, L. V., & Demyantseva, Y. V. (2019). Chondrogenic differentiation of mesenchal stem cells of the rabbit depending on the type of the cultural system. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 20(2), 250-260. https://doi.org/10.36359/scivp.2019-20-2.32