Histological and histochemical changes in dogs' kidneys for isoniazid poisoning

  • H. I. Kotsyumbas Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Lviv, Ukraine
  • N. P. Vretsona Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Lviv, Ukraine
Keywords: dog, kidneys, microstructural changes, poisoning, isoniazid, histology, histochemistry


The article presents the results of macroscopic, histological and histochemical studies of dogs' kidneys for isoniazid poisoning.

The path-anatomical section of 19 corpses of dogs of all ages and breeds has been carried out. Kidney fragments for morphological research have been selected. The kidney samples were fixed in a 10% aqueous solution of neutral formalin, Carnoua and Buena liquids. Dehydration was carried out in ethanol of increasing concentration and poured into paraffin. The slices were made on a microtome MC-2 in a thickness of 7 microns, stained with hematoxylin and eosin, according to the McManus and Pearl methodology. The produced histoplasts were examined under a light microscope Leica DM 2500 (Switzerland), photo-confirmed with the Leica DFC450C camera using the Leica Application Suite Version 4.4 software.

Macroscopic kidney blood filling, beanlike form, increased in volume. The surface of the organ is smooth, the fibrous kidney capsule was removed easily, in the cut border between the cortical and cerebrospinal fluid is expressed. The brilliant substance is colored in brownish-cherry color, while the cerebellum is a reddish-pinkish tint. Venous blood flowed from the cut surface of the cortical substance.

For histological examination in the kidneys it was determined: hyperemia, stasis, diapedeous hemorrhage, hemolysis of erythrocytes, which was accompanied by the deposition of hemosiderin granules and bilirubin in the epithelium of the tubules and macrophages; plasma penetration and accumulation of glycoproteins in the walls of the glomerular capillaries, Shumlyansky-Bowman capsules, arterioles, and in stromal elements of interstitium; in proximal convoluted tubules of the kidneys, hydropsical dystrophy and necrobiosis prevailed, indicating the development of necrotic tubulonephrosis. The revealed microstructural changes of the kidneys of dead dogs were not of the same type in the structures of the cortical and cerebral matter, but there was a general progressive violation of hemodynamics and destruction of cellular elements.


Toxicological profile for hydrazines U.S. Department of Health and Human Services Public Health Service Agency for Toxic Substances and Disease Registry. September 1997. P. 15–16.

Meyer, H., & Mally, J. (1912). On hydrazine derivatives of pyridine carbonic acids. Monatshefte Chemie verwandte Teile anderer Wissenschaften, 33, 393–414.

Berezhnoy, R. V., Gribov, V. M., & Denkovskiy, A. R. (1980). Rukovodstvo po sudebno-meditsinskoy ekspertize otravleniy. M. Meditsina, 404–405 (in Russian).

Chekman, I. S., Peleschuk, A. P., & Pyatak, O. A. (1986). Spravochnik po klinicheskoy farmakologii i farmakoterapii. Kiev «Zdorov’ya», 649–650 (in Russian).

Kutsenko, S. A. (2012). Osnovyi toksikologii. Sankt-Peterburg, 367–368 (in Russian).

Korzhevskiy, D. E., Gilyarov, A. V. (2010). Osnovyi gistologicheskoy tehniki. SPb. SpetsLit, 16–20 (in Russian).

Pirs, Je. (1962). Gistohimija teoreticheskaja i prikladnaja. M.: Inostr. lit. (in Russian).

Kotsyumbas, G. I., & Vretsona, N. P. (2018). Gistologichni ta gistohimichni zminy v pechintsi sobak za otruennya izoniazidom. Naukovyi visnyk LNUVMB imeni S. Z. Gzhytskogo, 20 (92), 197–202 (in Ukrainian).

Luzhnikov, E. A., & Kostomarova, L. G. (1989). Ostrye otravlenija. M.: Medicina, 151–153 (in Russian).

How to Cite
Kotsyumbas, H. I., & Vretsona, N. P. (2019). Histological and histochemical changes in dogs’ kidneys for isoniazid poisoning . Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 20(2), 238-246. https://doi.org/10.36359/scivp.2019-20-2.30