The study of pathological effects of Aspergillus Flavus field isolate on clinical and biochemical parametrs of the estonian quail organism

  • O. Orobchenko National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine", Kharkiv, Ukraine
  • M. Romanko National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine", Kharkiv, Ukraine
  • M. Yaroshenko National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine", Kharkiv, Ukraine
  • I. Gerilovich National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine", Kharkiv, Ukraine
  • O. Kutsan National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine", Kharkiv, Ukraine
Keywords: Aspergillus Flavus, quail, weight coefficients, clinic, micobiot, biochemical parameters, toxicity

Abstract

The purpose of the work was to determine the pathological effects of Aspergillus flavus field isolate on the clinical and biochemical state of the organism of quail of an Estonian breed 145 days age in the subchronic oral intake with feed. By analogy principle, birds were divided into 4 groups (n=16): one control and 3 experimental ones. The birds of the control group got full-fodder feed; І, ІІ and ІІІ experimental groups – feeds, artificially contaminated by the suspension of A. flavus spores in the amount of ~ 60,000, ~ 150,000 and ~ 300,000 spores per 1 gram of feed for 28 days, respectively. On the 14th, 21th, 28th days after the start and on the 7th day after completing the treatment, bird euthanasia and the selection of poultry organs and blood were carried out for further mycological and biochemical studies.

According to the results of the research, it has been established that prolonged treatment by spores of A. flavus with contaminated feed causes a gradual (on the 21st and 28th day) changes in the clinical and biochemical parameters of the bird. The coefficients of the weight of the internals of quail were determined. A decrease in the relative weight of the muscular stomach was established on the 21th days of the experiment in the birds of the experimental groups I and II and on the 7th day after completing the treatment by the contaminated feed – I; II and III groups (p<0.01; p <0.05) respectively. The registered decrease in the relative weight of the liver on the 28th day in the bird of the experimental group II and on the 7th day after the end of its feeding is III group (p<0.01; p<0.05), respectively. In determining the degree of contamination of target organs, it has been established that A. flavus growth in the lungs, muscular stomach and colon of quail is dose-dependent. Mechanism of pathological action A. flavus, the spores of which were artificially contaminated with food, in the quail organism is the gradual development of immune (leukocytosis, reduction of hemoglobin, excessive formation of toxic proteins – CIC and serumukoids, along with hypertension of ALT, P <0,05) and hepatotoxic reactions (raising the level of total proteins and creatinine, hypotensive of AST; P <0.05), the severity and irreversibility of which depends on the number of spores. The revealed metabolic changes in the bird organism were significantly expressed in the case of A. flavus contamination in the amount of ~ 300,000 spores per gram of feed (III experimental group), and did not recover even 7 days after the termination of its treatment.

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Published
2019-09-18
How to Cite
Orobchenko, O., Romanko, M., Yaroshenko, M., Gerilovich, I., & Kutsan, O. (2019). The study of pathological effects of Aspergillus Flavus field isolate on clinical and biochemical parametrs of the estonian quail organism. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 20(2), 200-216. https://doi.org/10.36359/scivp.2019-20-2.26