The study of pathological effects of Aspergillus Flavus field isolate on clinical and biochemical parametrs of the estonian quail organism
The purpose of the work was to determine the pathological effects of Aspergillus flavus field isolate on the clinical and biochemical state of the organism of quail of an Estonian breed 145 days age in the subchronic oral intake with feed. By analogy principle, birds were divided into 4 groups (n=16): one control and 3 experimental ones. The birds of the control group got full-fodder feed; І, ІІ and ІІІ experimental groups – feeds, artificially contaminated by the suspension of A. flavus spores in the amount of ~ 60,000, ~ 150,000 and ~ 300,000 spores per 1 gram of feed for 28 days, respectively. On the 14th, 21th, 28th days after the start and on the 7th day after completing the treatment, bird euthanasia and the selection of poultry organs and blood were carried out for further mycological and biochemical studies.
According to the results of the research, it has been established that prolonged treatment by spores of A. flavus with contaminated feed causes a gradual (on the 21st and 28th day) changes in the clinical and biochemical parameters of the bird. The coefficients of the weight of the internals of quail were determined. A decrease in the relative weight of the muscular stomach was established on the 21th days of the experiment in the birds of the experimental groups I and II and on the 7th day after completing the treatment by the contaminated feed – I; II and III groups (p<0.01; p <0.05) respectively. The registered decrease in the relative weight of the liver on the 28th day in the bird of the experimental group II and on the 7th day after the end of its feeding is III group (p<0.01; p<0.05), respectively. In determining the degree of contamination of target organs, it has been established that A. flavus growth in the lungs, muscular stomach and colon of quail is dose-dependent. Mechanism of pathological action A. flavus, the spores of which were artificially contaminated with food, in the quail organism is the gradual development of immune (leukocytosis, reduction of hemoglobin, excessive formation of toxic proteins – CIC and serumukoids, along with hypertension of ALT, P <0,05) and hepatotoxic reactions (raising the level of total proteins and creatinine, hypotensive of AST; P <0.05), the severity and irreversibility of which depends on the number of spores. The revealed metabolic changes in the bird organism were significantly expressed in the case of A. flavus contamination in the amount of ~ 300,000 spores per gram of feed (III experimental group), and did not recover even 7 days after the termination of its treatment.
Arroyo-Manzanares N. Mycotoxin Analysis: New Proposals for Sample Treatment // Advances in Chemistry. 2014 (2014), Article ID 547506, 12 р. Режим доступу : www.hindawi.com/journals/ac/2014/547506/
Martins T. Determination of quinolones and fluoroquinolones, tetracyclines and sulfonamides in bovine, swine andpoultry liver using LC-MS/MS // Food Additives and Contaminants – Part A Chemistry, Analysis, Control, Exposure and Risk Assessment. 2015. Vol. 32(3). Р. 1-9.
Paulsch W. E., Paulsch E. A., Egmond H. P. Van Liquid chromatographic determination of aflatoxins in feedstuffs containing citrus pulp : научное издание / Walter E. // J. Assoc. Offic. Anal. Chem. 1988. Vol. 71, № 5. P. 957-961.
Zakonodatel`no-normatyvnue akty Evropejskogo Soyuza (ES) v otnosheny`y` py`shhevoj promushlennosty`. 97 s. Rezhy`m dostupu: http://www.icc-iso.ru/upload/information_system_27/6/1/0/item_610/Zakonodatelno_normat_akty_ES.pdf [in Russian].
Yaroshenko, M. O., Kuczan, O. T. & Orobchenko, O. L. (2018) Monitory`ng kormiv dlya VRX molochnogo napryamu produkty`vnosti na nayavnist` plisenevy`x mikromicetiv u gospodarstvax pivnichno-sxidnogo regionu Ukrayiny`. Vetery`narna biotexnologiya. 32 (2). S. 602-610. [in Ukranian].
Axmadushen, R. A. & Kanarsky`j, A. V. (2007) My`kotoksy`nu – kontamy`nanu kormov. Vestny`k Kazanskogo texnology`cheskogo uny`versy`teta. N2. S.88-103. [in Russian].
Sutton, D., Fotergill, M. & Rinaldi, M. (2001). Opredelitel patogennykh i uslovno patogennykh gribov [The determinant of pathogenic and conditionally pathogenic fungi]. Moscow: Mir [in Russian].
Aspergillus flavus // Mycobank.org. – CBS-KNAW Fungal Biodiversity Center Utrecht, 2016.
Tell L. A. Aspergillosis in mammals and birds: impact on veterinary medicine. Med Mycol. 2005 May. 43 Suppl. 1. S 71-73.
Bilay, V.I. & Koval, E.Z. (1988). Aspergilly: opredelitel [Aspergillus: determinant]. Kiev: Naukova Dumka [in Russian].
Kashky`n, P. N. (1979) Opredely`tel` patogennuch, toksy`gennuch y` vrednuch dlya cheloveka gry`bov / P. N. Kashky`n, M. K. Xoxryakov, A. P. Kashky`n. Leny`ngrad: Medy`cy`na, 270 s. [in Russian].
Bolshoj praktykum po mykrobyologyy. (1962). Pod red. prof. G. L. Sy`mbera. M. Vusshaya shkola. S.106-112. [in Russian].
Obrazhej, A. F., Pogrebnyak, L. I. & Korzunenko O. F. (1998) Metody`chni vkazivky` po sanitarno-mikologichnij ocinci ta polipshennyu yakosti kormiv : metod. vkazivky`, zatverdzheni Derzh. departamentom vet. medy`cy`ny` Ministerstva APK Ukrayiny` N15-14/73 vid 06 bereznya 1998. Ky`yiv. 107 s. [in Ukranian].
European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Council of Europe. Strasbourg, 1986. 53 p.
Stattya 26 Zakonu Ukrayiny` N5456-VI vid 16.10.2012 r. (2012) «Pro zaxy`st tvary`n vid zhorstokogo povodzhennya». [in Ukranian].
Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes // Officіal Journal of the European Communities L 358. 1986. Р. 1-29.
Zharov, A. V., Strelnikov A. P. & Ivanov I. V (2003) Vskrytiye i patomorfologicheskaya diagnostika bolezney zhivotnykh [Autopsy and pathological diagnosis of animal diseases]. Moscow: KolosS [in Russian].
Men`shy`kov, V. V. (1987) Laboratornue metodu issledovany`ya v klinike: Spravochny`k. M.: Medy`cy`na. 386 s. [in Russian].
Vlizlo, V. V., Fedoruk, R. S. & Raty`ch, I. B. (2012) Laboratorni metody` doslidzhen` u biologiyi, tvary`nny`cztvi ta vetery`narnij medy`cy`ni : dovidny`k. / Pid red. Vlizla V. V. L`viv, SPOLOM. 764 s. [in Ukranian].
Levchenko, V. I. ta in. (2002) Vetery`narna klinichna bioximiya. / Pid red. Levchenka V. I. Bila Cerkva. BDAU. 400 s. [in Ukranian].
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