Immunobiological properties of field cultures

  • I. A. Biben Dnipro State Agrarian-Economic University, Dnipro, Ukraine
  • A. A. Sosnitska Dnipro State Agrarian-Economic University, Dnipro, Ukraine
  • E. V. Udovitsky Dnipro State Agrarian-Economic University, Dnipro, Ukraine
  • V. V. Zazharsky Dnipro State Agrarian-Economic University, Dnipro, Ukraine
Keywords: atypical mycobacteria, M. VACCAE, immunostimulation, probiotic cultures, biopropod, P. MULTOCIDA SB. GALLICIDA, biomaterial


When conducting a simultaneous intradermal allergic study, 12 heads of cattle found that they were sensitized with atypical mycobacteria allergens. The animals were clinically healthy and were located in an area that was safe for infectious diseases. Bacteriological examination of cow manure with pre-sowing treatment of the biomaterial according to the method of A. P. Alikaeva succeeded in isolating cultures of mycobacteria, which, by a set of characteristics, were identified as M. phlei; M. flavenscen; M. vaccae; M. terra.

In the study of sensitizing and pathogenic properties in guinea pigs, it was found that all isolates have a pronounced sensitizing potential and do not cause pathological changes in the organism of infected animals. The highest intensity of allergic reactions was observed 60 days after infection, followed by a decline and release of the body from atypical mycobacteria. Isolates in the body of white mice increased nonspecific resistance. that was shown during infection with DCL P. multocida sb. gallicida.

The number of pasteurellosis 4-5 DL50 was used as a direct infection control. To increase non-specific resistance, preventive administration of increasing doses of atypical mycobacterial cultures from 0.01 mg to 100.0 mg of raw pressed bacterial mass per animal was used. As a result, the culture of M. vaccae showed the greatest protective activity, the smallest — M. phlei.

The cultures of atypical mycobacteria M. flavenscen and M. terra occupied an intermediate position, showing an average level of protective activity. Based on the conducted experiments, the biological characteristics of M. vaccae were recognized as the most suitable culture for use as a probiotic microorganism in the creation of a complex biological product for stimulating the immunobiological mechanisms of an animal organism


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How to Cite
Biben, I. A., Sosnitska, A. A., Udovitsky, E. V., & Zazharsky, V. V. (2019). Immunobiological properties of field cultures. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 20(2), 174-182.