Effect of ethanol plant extracts on Staphylococcus Epidermidis, Staphylococcus Aureus

  • V. V. Zazharskyi Dnipro State Agrarian and Economic University, Dnipro, Ukraine
  • P. O. Davydenko Dnipro State Agrarian and Economic University, Dnipro, Ukraine
  • O. M. Kulishenko Dnipro State Agrarian and Economic University, Dnipro, Ukraine
  • I. V. Borovik Dnipropetrovsk Regional State Laboratory of the State Service of Ukraine for Food Safety and Consumer Protection, Dnipro, Ukraine
  • V. V. Brygadyrenko Oles Honchar Dnipro National University, Dnipro, Ukraine
Keywords: antibacterial activity, tincture, Staphylococcus Epidermidis, Staphylococcus Aureus

Abstract

The emergence of multiresistant strains of Staphylococcus epidermidis, Staphylococcus aureus that are difficult to antibiotics and cause severe lesions of soft tissues, sepsis, and complicated surgical pathology are recognized as the one of problems of current infectious diseases of animals and humans. One of challenges in pharmacognosy is the search for alternative sources of antibacterial substances with an exhaustive resource of antibiotics of fungal origin. The use of raw medicinal plants is quite promising in this regard. The tendency of scientific research of recent decade reveals a promising range of plants of a number of families, which typically contents certain active substances (phytoncides, saponins, alkaloids, glycosides, tannins, essential oils etc.).

The goal of the work was to establish the antibacterial effect of plant infusions on reference cryogenic strains of Staphylococcus epidermidis, Staphylococcus aureus in vitro.

Herbal material of 50 species (seeds, grass, shoots, leaves, compound fruit, peel) obtained at different periods of the growing season was used for investigation. The material was classified, dried, and grounded. Samples of 1 g were poured with 5 cm3 of 96 % ethanol and were kept it over three weeks in a dry cold place. The obtained alcohol infusion was filtered with sterile multi-layer gauze disc filters. Before the discs were put on the surface of agar with inoculation of the corresponding culture, they were dried in a sterile laminar box under ultraviolet rays. Antibacterial activity of various tinctures was determined by the disk diffusion method in agar with the measurement of the diameter of the growth suppression zone of the culture using a template ruler.

Concerning the above mentioned point, herein, we report the results of the use of tinctures Staphylococcus epidermidis, Staphylococcus aureus in vitro. Obtained data has been systematized, summarized and evaluated.

The paper presents the results of the effectiveness of phytopreparations on Staphylococcus epidermidis, Staphylococcus aureus in vitro. The antibacterial effect of plant tinctures of Cephalotaxus harringtonia, Hedera helix, Geranium sanguineum on cryogenic strains Staphylococcus epidermidis, Staphylococcus aureus. We consider it possible to recommend the investigated extracts of Cephalotaxus harringtonia, Hedera helix, Geranium sanguineum for further research in the fight against polyresistant strains of the above-mentioned microorganisms.

The obtained results give grounds to recommend herbal tinctures to combat multi-resistant strains of Staphylococcus epidermidis, Staphylococcus aureus.

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Published
2019-09-18
How to Cite
Zazharskyi, V. V., Davydenko, P. O., Kulishenko, O. M., Borovik, I. V., & Brygadyrenko, V. V. (2019). Effect of ethanol plant extracts on Staphylococcus Epidermidis, Staphylococcus Aureus. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 20(2), 154-161. https://doi.org/10.36359/scivp.2019-20-2.20