Antimicrobial activity of danofloxacin, effectiveness and safeness of a new antimicrobial preparation based on its basis for treatment of calves with respiratory bacterial infections

  • T. Stetsko State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
  • I. Kocjumbas State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
  • V. Muzyka State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
  • N. Lisova State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
  • O. Pyatnichko State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
  • L. Ostrovska State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
Keywords: fluoroquinolones, danofloxacin, cattle, calves, respiratory infections, sensitivity of microorganisms, therapeutic efficiency, safety

Abstract

In the article results of investigation the sensitivity of bacteria-isolates, pathogens of acute bacterial infection of respiratory tract in young cattle, to danofloxacin, the fluoroquinolone antibiotic of the "third generation", present. So, the minimum inhibitory concentration of danofloxacin for Staphylococcus aureus isolates is 0.32 ± 0.052 μg/ml (n = 20) and Streptococcus pneumoniae isolates is 0.29 ± 0.064 μg/ml (n = 10) that indicates a high degree of sensitivity of pathogens of respiratory infection  in cattle to danofloxacin.

Using of a new antimicrobial drug on the basis of danofloxacin Danoflox 2.5% (solution for injection) caused a clinical recovery in calves with acute bacterial respiratory infection, what was confirmed by the results of a general analysis of the blood of diseased animals after antibiotic therapy. After treatment, changes in the parameters of the general blood test were observed: the concentration of hemoglobin and the number of red blood cells significantly increased, the number of "white" blood cells decreased, the percentage of lymphocytes decreased to norm, the number of banded and segmented neutrophils and eosinophils came to norm. After antibiotic therapy, the level of total serum protein, serum albumin, α-globulins in proteinogram, normalization of the level of γ-globulins is observed. A slight increase in the activity of ALT and the content of creatinine, however, these indicators did not go beyond the limits of physiological norm. Investigation of  blood immunological parameters showed an absence of negative influence of the preparation on immune protection. 

Side reactions or unacceptable phenomenons in animals during and after using of Danoflox 2.5 % have not been observed. It indicates a high level of preparation safety in the use of young animals in the recommended dosage.

References

Edwards A. J. Respiratory Diseases of Feedlot Cattle in the Central USA / A. J. Edwards // Bovine Practitioner. – 1996. – Vol. 30. – P. 5-7.

Galyean M. L. Interaction of Cattle Health / M.L Galyean, L.J. Perino and G.C. Duff / Immunity and Nutrition // Journal of Animal Science – 1999. – Vol. 77. – P. 1120-1134.

Trends in Mortality Ratios Among Cattle in US Feedlots / G. H. Loneragan, D. A. Dargatz, P. S. Morley, M. A. Smith // Journal of the American Veterinary Medical Association. – 2001. – Vol. 219. – P. 1122-1127.

Regional management practices and prevalence of bovine respiratory disease in California’s preweaned dairy calves / G. U. Maier, W. J. Love, S. A. Dubrovsky et al. // J. Dairy Sci. – 2019. – Vol. 102. – P. 1–14.

The epidemiology of bovine respiratory disease: What is the evidence for predisposing factors? / J. D. Taylor, R. W. Fulton, T. W. Lehenbauer et al. // Canadian Veterinary Journal, Ottawa– 2010.– Vol. 51, N. 10. – Р. 1095-1102.

Cusack P. M. V. The medicine and epidemiology of bovine respiratory disease in feedlots. / P.M.V. Cusack, N. P. McMeniman, and I. J. Lean. // Aust. Vet. J.– 2003.– Vol. 81. – P. 480-487.

Bacterial pathogens of the bovine respiratory disease complex. / D. Griffin, M. M. Chengappa, J. Kuszak, D. S. Mcvey // Veterinary Clinics of North America: Food Animal Practice, Philadelphia. – 2010.– Vol. 26, N. 2. – P. 381-394.

Alexander B. H. Risk factors for lower respiratory tract disease in a cohort of feedlot cattle / B. H. Alexander, M. D. MacVean and Salman // JAVMA. – 1989. – Vol. 95(2). – P. 207-211.

Yates W. D. A review of infectious bovine rhinotracheitis, shipping fever pneumonia and viral-bacterial synergism in respiratory disease of cattle / W. D. Yates // Canadian Journal of Comparative Medicine. – 1982. – Vol. 46. – P. 225-263.

Ames T. R. The Bronchopneumonias (Respiratory disease complex of cattle, sheep and goats)./ T. R. Ames, J. C. Baker, S. E. Wikse: In Smith B P (eds) // Large Animal Internal Medicine. Mosby Inc, St Louis, 2002. – Р. 551-570.

Hamdy A. H. Investigation of nasal microflora of feedlot calvesbefore and after weaning / A. H. Hamdy, A. L. Trapp // American Journal of Veterinary Research. – 1967. – Vol. 28. – P. 1019-1025.

A search for new microorganisms in calf pneumonia by the inoculation of gnotobiotic calves. / L. H. Thomas, R. N. Gourlay, E. J. Stott et al. // Research in Veterinary Science. – 1982. – Vol. 33. – P. 170-182.

Barragry T. B. Therapy of bovine respiratory disease. / T. B. Barragry // Veterinary Drug Therapy. Lea & Febiger, Philadelphia, 1994. – P. 780-810.

Stetsko T. I. Zasady efektyvnoi antybiotykoterapiyi u veterynarniy medytsini / T. I. Stetsko // Veterynarna biotechnologiya. – 2008. – № 13 (1). – 194–203. (in Ukrainen)

Papich M. G. Fluoroquinolone antimicrobial drugs / M. G. Papich, J. E. Riviere //In: Adams HR, editor. Veterinary Pharmacology and Therapeutics, 8th ed. Ames: Iowa State University Press, 2001. – Р. 898-912.

Hooper D. C. Mechanism of quinolone action and bacterial killing / D.C. Hooper, J.S. Wolfson: in “Quinolone Antimicrobial Agents”, 2nd ed., Eds. Amer. Soc. For Microbiol., Washington, 1993. – P. 482-512.

Smith L. T. Chemistry and mechanisms of action of the quinolone antibacterials / L. T. Smith, C. S. Lewin //in “Quinolones”, Ed. Andriole V., Academ. Press, 1988. – P. 23–42.

Stetsko T. I. / Krytychno vazhlyvi antymicrobni preparaty dlya veterynarnoi medytsiny / T. I. Stetsko, V. P. Muzyka, V. P. Gunchak // Naukovyi visnyk LNUVMB imeni S. Z. Gzhitskogo. – 2018. – Т. 20, № 87. – 19-26. (in Ukrainian)

Stetsko T. I. Rezistentnist` do ftorhinoloniv: pochodzhennya, evolutsiya, klinichne znachennya ta shlyahi podolannya / T. I. Stetsko // Biologiya tvaryn. – 2005. – 7, № 1-2. – 51-63. (in Ukrainian)

Brown S. A. Fluoroquinolones in animal health / S. A Brown // J Vet Pharmacol Ther. – 1996. – Vol. 19. – P. 1-14.

Martinez-Martinez L. Quinolone resistance from a transferable plasmid / A. Pascual, G. A. Jacoby // Lancet. – 1998. – Vol. 351. – Р. 797-799.

Gorbatuk B. I. Metodychni rekomendacii do laboratornyh zanyat`dyagnostyki ta doslidzhennya zagal`nogo stanu organizmu tvaryn. – Львів, 2004. – 72 с.

Stetsko T.I. et. al. Metodychni vkazivky po vyznachenniu chutlyvosti microorganismiv do antymikrobnych preparativ metodom dyfusii v agar za dopomogoju standartnych dyskiv z antybiotykamy (zatvedzheni naukovo-tehnichnoju radoju DDVM Ukrainy 20.12.2007 р.) – Lviv, 2010. – 12. (in Ukrainian)

National Committee for Clinical Laboratory Standards. 2004. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; informational supplement. M31-S1. National Committee for Clinical Laboratory Standards, Wayne, PA.

Kosenko M. V. et. al. Metodychni vkazivky po vyznachenniu bacteriostatychnoi ta bactericidnoi koncentracii antybacterialnych preparativ metodom serijnych rozveden` (zatvedzheni naukovo-tehnichnoju radoju DDVM Ukrainy Ministerstva agropolityky Ukrainy 19.12.2002 р.) – Kyiv, 2003. – 7. (in Ukrainian)

Kompleksna otcinka vlyvu veterynarnych preparativ na morphofunccinalnyi stan imunnoi systemy: Metodychni rekomendacii / I. Kocjumbas, G. Kocjumbas, Golubyi E. et. al. – Львів, 2009. – 63. (in Ukrainian)

Klinichni doslidzhennya veterynarnych preparativ ta kormovych dobavok /

I. Kocjumbas, I, Bisjuk, B. Gorzheev et. al; za red. I. Kocjumbasa. – L.: ТОV Vyd. dim «SAM», 2013. – 252. (in Ukrainian)

Laboratorni metody doslidzhennya u biologiyi, tvarynnytstvi ta veterynarniy medytsini: dovidnyk / V. V. Vlizlo, R. S. Fedoruk, I. B. Ratych et. al; za red. V. V. Vlizla. – Lviv: Spolom, 2012. – 764. (in Ukrainian)

Statistical principles for veterinary clinical trials. CVMP/EWP/81976/2010; Demonstration of efficacy for veterinary medicinal products containing antimicrobial substances. CVMP/627/2001.

Published
2019-09-18
How to Cite
Stetsko, T., Kocjumbas, I., Muzyka, V., Lisova, N., Pyatnichko, O., & Ostrovska, L. (2019). Antimicrobial activity of danofloxacin, effectiveness and safeness of a new antimicrobial preparation based on its basis for treatment of calves with respiratory bacterial infections. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 20(2), 126-137. https://doi.org/10.36359/scivp.2019-20-2.17