• I. A. Biben Dnipro State Agrarian-Economic University 25, S. Efremova str., Dnipro, 49600, Ukraine
  • O. I. Sosnitskyi Dnipro State Agrarian-Economic University 25, S. Efremova str., Dnipro, 49600, Ukraine
  • V. V. Zazharsky Dnipro State Agrarian-Economic University 25, S. Efremova str., Dnipro, 49600, Ukraine
  • A. O. Sosnitska Dnipro State Agrarian-Economic University 25, S. Efremova str., Dnipro, 49600, Ukraine


Prokaryotes of the genus Mycobacterium occupy an important evolutionary and historical place in the system of microorganisms of prokaryotic and eukaryotic organization in the Earth's biosphere. These are the most ancient microorganisms that have passed a long historical path of mutual adaptation with the evolving biosphere over billions of years. Antiquity of origin is very important in the process of developing a stable and at the same time adaptive genomic apparatus, which provides genetic information support for the very diverse physiological needs of a carrier of a given genotype in the constantly changing conditions of the environment of existence and creating competitive advantages over similar carriers of gene programs in the limited space of a specific ecosystem.

A significant species diversity of prokaryotes of the genus Mycobacterium is a consequence of the wide divergence of the ancestral ancestor species of the sapronous type in the process of soil formation from lithotrophic forms to the highest degree of bioparasitism in the form of highly specialized infectious pathogens with pronounced pathogenicity, invasiveness and the potential ability to take root in an internal macroorganism and an extremely effective antagonist the immune system of supervision over the genetic homeostasis of a macroorganism with a paradigm of tolerance to its genetically own and categorical intolerance to carriers of geneticaly foreign information.

Only a small number of mycobacterial species have adapted to the internal environment of eukaryotic macroorganisms as a result of the suppression of the protective mechanisms of the lymphoid system by pathogenic factors. The path of symbiotic relationships, mutually beneficial interpopulation, turned out to be much more effective. As a result, a dynamic microbial organ arose - the microbiota of the large intestine, inhabited by indigenous and transient microbionts, among which Mycobacterium vaccae occupy an important place.

Ecological strains "K", "N", "Gk", "Gn" of Mycobacterium vaccae were isolated and identified by conventional bacteriological methods. Examined their basic properties. The strains possessed morpho-tinctorial, cultural and biochemical properties characteristic of the species, were apathogenic for laboratory animals when administered parenterally, caused short-term sensitization to mycobacterial antigens and induced antitilogenesis. In broiler chickens on fattening with oral feeding of live culture, they stimulated metabolism, increased weight gain and increased the protective properties of the nonspecific link of the immune reactivity of the macroorganism.

Strains of atypical mycobacteria were isolated on the territory of one poultry farm and had similar biocharacteristics; they are suitable for use as probiotics to increase nonspecific resistance, stimulate the physiological functions of the macroorganism, increase weight gain and improve feed payment.


Apatenko, V.M. & Kopietskiy, V.F. (2008). Prevolyutsiya mikrobiv yak epizootologichniy chinnik. Vet. meditsina Ukrayini, 4, 11-12. [in Ukrainian].

Biben, I.A., Sosnitska, A.O., Zazharskiy, V.V., Sosnitskiy, O.I. (2019). Epizootichniy shtam SA-18 Pasteurella multocida dlya vigotovlennya tsilnoklitinnogo bakterialnogo inaktivovanogo antigenu u skladi inaktivovanoyi ad’yuvant-vaktsini proti pasterelozu ssavtsiv ta ptitsi, indukovanogo Pasteurella multocida serovarom A: pat. 135161 Ukraina. byul. 12, 4. [in Ukrainian].

Domaradskiy, I.V. (1997). Virulentnost mikobakteriy kak funktsiya adaptatsii. ZhMEI, 4, 16-20. [in Russian].

Gorbatyuk, B.I. (2004). Metodichni rekomendatsiyi z diagnostiki ta doslidzhennya zagalnogo stanu organIzmu tvarini. Lviv, 72. [in Ukrainian].

Guarner, F, Khan, G.A., Garisch, J. et al. (2008). Probiotiki i prebiotiki [Elektronnyiy resurs] World Gastroenterology Organisation. Rezhim dostupa: http//

Kalashnik, M.V. (2017). Epizootologichniy monitoring ta udoskonalennya laboratornoyi diagnostiki tuberkulozu velikoyi rogatoyi hudobi: avtoref. dis. ... kand. vet. nauk. Harkiv, 20. [in Ukrainian].

Khan, M., Raoult, D, Richeta, H. (2007). Growth-promoting effects of single-dose intragastrically administered probiotics in chickens. British Poultry Science. 48(6), 732-735.

Kislichkina, A.A., Stepanshina, V.I., Shemyakin, I.G. (2009). Mikobakteriozyi, Problemyi tuberkuleza i bolezni legkih. 5, 3-9. [in Ukrainian].

Kotsyumbas, I.Ya., Bisyuk, I.Yu., Gorzheev, V.M, Malik, O.G. et al. (2013). Klinichni doslidzhennya veterinarnih preparativ ta kormovih dobavok; za red. I.Ya. Kotsyumbasa. Lviv, 252. [in Ukrainian].

Litvinov, V.I., Makarova, M.V., Krasnova, M.A. (2008). Netuberkuleznyie mikobakterii. Moskva, 256. [in Russian].

Makarova M.V. (2010). Vyidelenie i identifikatsiya netuberkuleznyih mikobakteriy u patsientov ftiziatricheskih uchrezhdeniy: avtoref. dis. … d-ra biol. nauk. Moskva, 48. [in Russian].

Magee, J.G., Ward, A.C. (2015). Mycobacterium. Bergey’s Manual of Systematics of Archaea and Bacteria. – Chichester, UK: John Wiley & Sons, 1-84.

Martin-Casabona, N., Bahrmand, A,R, Bennedsen, J. (2004). Non-tuberculous mycobacteria: patterns of isolation, a multy-country retrospective survey. The international journal of tuberculosis and lung disease the official journal of the International Union against Tuberculosis and Lund Disease. 8,10. 1186-1193.

Metodicheskie rekomendatsii po diagnostike zabolevaniy selskohozyaystvennyih zhivotnyih i ptitsyi s ispolzovaniem serologicheskih reaktsiy. (2008). Vladimir. [in Russian].

Michael, D.I. (1998). Nontuberculous mycobacteria (ntm). Philadelphia: WB Saunders company, 1513-1528.

Murray, P.R., Rosenthal, K.S., Pfaller, M.A. (2005). Medical microbiology. Elsevier Mosby, 963.

Naymanov, A.H., Ustinova, G.I., Tolstenko, N.G. et al. (2005). Netuberkuleznyie (atipichnyie) mikobakterii i ih sensibiliziruyuschee znachenie. Veterinariya i kormlenie. 1, 19-21. [in Russian].

Novotny, L., Dvorska, L., Lorencova, A. et al. (2004). Fish: a potential source of bacterial pathogens for human beings. Vet. Med. – Czech. 49, 9. 343-358.

O’Brien, M.E.R., Anderson, H., Kaukel, E. & et al. (2004). SRL 172 (Killed Mycobacterium vaccae) in addition to standard chemotherapy improves quality of life, without affecting survival, in patients with advanced non-small-cell ling cancer: phase III results. Annals of oncology. 15, 6. 906-914.

Reber, S.O., Siebler, P.H., Donner, N.C. et al. (2016). Immunization with a heat-killed preparation of the environmental bacterium Mycobacterium vaccae promotes stress resilience in mice. The Rockefeller University, New York. 113, 22. 3130-3139.

Roberts, C.A. & Manchester, K. (2005). The archaeology of disease. Ithaca, Unated States : Cornell University Press, 352.

Runyon, E.H. (1965). Typical mycobacteria: their classification. The American review of respiratory disease.91, 288-289.

Skripnik, A.V. (2007). Molekulyarno-genetichna diferentsIatsIya mikobakteriy, vidIlenih v Ukrayini, ta yih fIiogenetichni vzaemozv’yazki: dis. … kand. vet. nauk: Harkiv. 150. [in Ukrainian].

Sosnitskiy, O.I. & Alekseeva, N.V. (2015). Viniknennya vognisch proliferativnogo netuberkuloznogo zapalennya v limfatichnih vuzlah vidgodivelnih pidsvinkiv. Naukovo-tehnichniy byulleten Derzhavnogo naukovo-tehnichnogo kontrolnogo Institutu veterinarnih preparativ ta kormovih dobavok i Institutu biologiyi tvarin. Lviv. 16, 2. 229-234. [in Ukrainian].

Vlizlo, V.V., Fedoruk, R.S., Ratich, I.B. et al. (2012). Laboratorni metodi doslidzhen u biologiyi, tvarinnitstvi ta veterinarniy meditsini: dovidnik; za red. V.V. Vlizla. Lviv: СПОЛОМ, 764. [in Ukrainian].

West, N.P., Pyne, D.B., Peake, J.M. et al. (2009). Probiotics, immunity and exercise: a review. Exers. Immunol. Rev. 15,(107). 107-126.

Yankovskiy, D.S. (2005). Mikrobnaya ekologiya cheloveka: sovremennyie vozmozhnosti ee podderzhaniya i vosstanovleniya. K., 362. [in Ukrainian].

Yaschenko, T.N., Mecheva, I.S. (1973). Rukovodstvo po laboratornyim issledovaniyam pri tuberkuleze. Moskva: Meditsina, 260. [in Russian].

Zavgorodniy, A.I. (1997).Vidi mikobakteriy, rozpovsyudzhenni v gospodarstvah Ukrayini ta yih epizootichne znachennya: avtoref. dis. ... d-ra vet. nauk. Harkiv, 32. [in Ukrainian].

Zavgorodniy, A.I. (2013). Naukove zabezpechennya protituberkuloznih zahodiv v Ukrayini. Vet. meditsina Ukrayini. 10, 15-16. [in Ukrainian].

How to Cite
Biben, I. A., Sosnitskyi, O. I., Zazharsky, V. V., & Sosnitska, A. O. (2021). BIOLOGICAL PROPERTY OF THE ECOLOGICAL CULTURES MYCOBACTERIUM VACCAE. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 22(1), 38-51.