EFFECTIVENESS OF MESENCHYMAL STEM CELLS IN UVEITIS IN DOGS, DEPENDING ON THE METHOD OF THEIR ADMINISTRATION

  • O. V. Shupyk National University of Life and Environmental Sciences of Ukraine
  • R. R. Bokotko National University of Life and Environmental Sciences of Ukraine
  • T. L. Savchuk National University of Life and Environmental Sciences of Ukraine
  • V. B. Danilov National University of Life and Environmental Sciences of Ukraine
  • L. V. Kladnytska National University of Life and Environmental Sciences of Ukraine
  • Yа. О. Kharkevych National University of Life and Environmental Sciences of Ukraine
  • O. S. Pasnichenko Odessa state Agrarian University
  • R. S. Blahyi Rohatyn State Agricultural College
  • N. I. Hraborenko Separate Structural Subdivision Nova Kakhovka College of Dmytro Motornyi Tavria State Agrotechnological University
  • Y. M. Krystyniak Sokolivska veterinary hospital
Keywords: TOTAL UVEITIS, DOGS, MESENCHYMAL STEM CELLS, EYE, TENITIUM SPACE, ANTERIOR CHAMBER OF THE EYE, POSTERIOR CHAMBER OF THE EYE, TISSUE.

Abstract

The article highlights the results of studies on spontaneous clinical cases of bacterial uveitis in dogs, using a slit lamp, sonographic and visual research methods, and after the introduction of mesenchymal stem cells in various ways. These research results make it possible to analyze and further study the effect of mesenchymal stem cells, when administered in various ways in total uveitis, and determine the most effective method in clinical cases in dogs.

Chronic total uveitis is an extremely severe late complication of ophthalmic surgery, and leads to slow functional and anatomical death of the eye. The use of antibacterial drugs for the Prevention of infectious complications often smooths out the clinical picture of progressive intraocular inflammation, contributing to an increase in its latent period. All this with particular relevance indicates the need for research as early as possible, and the specifics of the use of stem cells in eye diseases in dogs.

Our research on the restoration of eye tissues depends on many factors, on the balance between inflammatory mediators and their antagonists, which activate the synthesis of anti-inflammatory mediators. Also, it depends on the number of mesenchymal stem cells that act as a regulator of proliferation in damaged eye tissues and cause cyto-differentiation during cell regeneration and enhance their own antioxidant properties.

It was found that microbial contamination significantly deepens the consequences of damage to tissues and structures of the eye, which lead to acidosis of tissues, changes microcirculation and exit and destruction of cell membranes and prolongation of the inflammatory phase, namely, it determines the course and results of the reparative process. At the same time, we can talk not only about the recovery function of damaged tissue structures with the help of mesenchymal stem cells, but also the effect on the intensity of the inflammatory process, which significantly reduces the time of repair of eye tissues at the level of cells and tissues.

The data obtained can be used for new modern methods of treating many pathologies associated with eye tissue damage and the use of mesenchymal stem cells in reparative ophthalmology.

References

Astakhov, S.Iu. & Vokhmiakov, A.V. (2008). Endoftalmit: profilaktyka, diahnostyka, lechenye. Oftalmolohycheskye Vedomosty. 1. 36–45 [in Russian].

Barry, P., Gardner, S., Seal, D., Gettinby, G., Lees, F., Peterson, M., Revie, C. (2009). Clinical observations associated with proven and unproven cases in the ESCRS study of prophylaxis of postoperative endophthalmitis after cataract surgery. J Cataract Refract Surg. 35 (9). 1523–1531.

Dimarino, A.M., Caplan, A.I., Bonfield, T.L. (2013). Mesenchymal Stem Cells in Tissue Repair. Front Immunol. 4. 201.

Frolychev, I.A. & Pozdeeva, N.A. (2017). Posleoperacionnyj jendoftalmit. Obzor literatury. Prakticheskaja medicina. 110 (9). 192–195 [in Russian].

Koch, H.R, Kulus, S.C., Roessler, M. (2005). Corneal penetration of fluoroquinolones: aqueous humor concentrations after topical application of levofloxacin 0,5% and ofloxacin 0,3% eyedrops. J. Cataract Refract. Surg. 31. 7. 1377-1385.

Maalouf, F., Abdulaal, M., Hamam, R.N. (2012). Chronic Postoperative Endophthalmitis: A Review of Clinical Characteristics, Microbiology, Treatment Strategies, and Out comes. Int J Inflam. 313-248.

Meisler, D.M., Palestin, A.G., Vastine, D.V., Demartini, D.R., Murphy, B.F., Rein, W.J., Zakov, Z.N., Mahon, J.T., Cliffel, T.P. (1986). Chronic propionibacterium endophthalmitis after extracapsular cataract extraction and intraocular lens implanta tion Am. J. Ophthalmol. 102(6). 733– 739.

Nesterov, A.P. & Basynskyi, S.N. (1991). Novyi metod vvedenyia lekarstvennykh preparatov v zadnyi otdel tenonova prostranstva. Vestnyk oftalmolohyy. 5. 49-51 [in Ukrainian].

Savchuk, T.L., Mazurkevych, A.Y., Maliuk M.O., Kharkevych Yu.O. (2018). Biokhimichni zminy u syrovattsi krovi kroliv za eksperymentalnoho mekhanichnoho poshkodzhennia kistkovoi tkanyny pislia zastosuvannia alohennykh mezenkhimalnykh stovburovykh klityn. Naukovyi visnyk Natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrainy. Seriia: Veterynarna medytsyna, yakist i bezpeka produktsii tvarynnytstva. 2018. № 285. S. 240–251 [in Ukrainian].

Savchuk, T.L., Bokotko, R.R., Kharkevych, Yu.O., Mazurkevych, A.Y., Maliuk, M.O., Danilov, V.B., Blahyi, R.S., Braha, O.V. (2020). Makroskopichni zminy v eksperymentalno ushkodzhenii velykohomilkovii kisttsi kroliv za vvedennia alohennykh mezenkhimalnykh stovburovykh klityn riznymy sposobamy. NTB Derzhavnoho naukovo-doslidnoho kontrolnoho instytutu vetpreparativ ta kormovykh dobavok i Instytutu biolohii tvaryn 21. 1. 168-174 [in Ukrainian].

Stebnev, V.S., Skladchikova, N.Ju., Stebnev, S.D. (2008). Effektivnost kompleksnoj jendookuljarnoj hirurgii hronicheskogo jendoftal'mita v artifakichnom glazu. Vestnik Orenburgskogo gosudarstvennogo universiteta. 94 (12– 22). 118–122 [in Russian].

Weyenberg, W. & Vermeire, A. (2004). Effect of different sterilization method on the properties of bioadhesive powders and ocular minitablets, and clinical evaluation. Eur. J. Pharm. Sci. 23. 1. 68-71.

Published
2020-10-27
How to Cite
Shupyk, O. V., Bokotko, R. R., Savchuk, T. L., Danilov, V. B., Kladnytska, L. V., KharkevychY. О., Pasnichenko, O. S., Blahyi, R. S., Hraborenko, N. I., & Krystyniak, Y. M. (2020). EFFECTIVENESS OF MESENCHYMAL STEM CELLS IN UVEITIS IN DOGS, DEPENDING ON THE METHOD OF THEIR ADMINISTRATION. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 21(2), 219-229. https://doi.org/10.36359/scivp.2020-21-2.29