Morphological indicators of rat's blood under conditions of oxidation stress and for action of the food additives «Butaselmevit-plus»
Free radical oxidation plays an extremely important role in the development of many pathological processes. Poisoning of experimental animals with tetrachloromethane according to the morphological picture and biochemical changes close to the acute liver lesions of different etiologies in humans and animals. That is why in our work the classic model of damage of hepatocyte subcellular membranes has been used and the development of oxidation stress based on the use of tetrachloromethane. The purpose of the work was to study the morphological parameters of blood in rats under the conditions of oxidative stress and the action of the feed additive «Butaselem-plus». The studies were conducted on white, sexually-mature, young male rats of the Wistar line with a body weight of 180–200 g, which were kept on the standard diet of the institute vivarium of the State Scientific-Research Control Institute of Veterinary Preparations and Feed Additives. Animals were divided into three groups of 20 animals in each: 1st group (K) intact animals; Group 2 (D1) – rat, afflicted with tetrachloromethane; Group 3 (D2) – rat, lesions tetrachloromethane and which were used as feed additive «Butaselem-plus». Experimental intoxication in animals was carried out by two-time (after 48 h) of intravenous administration of tetrachloromethane in a dose of 0.1 ml per 100 g of body weight of the rat in the form of 50% of the oil solution. For the experimental group D2 for experimental toxicosis for 30 days fed a feed supplement «Butaselmeib plus» in a dose of 0.1 g per 100 g of body weight with food.
It was established that under conditions of intoxication with tetrachloromethane in rats, hematopoietic function of bone marrow is violated, which is manifested by a decrease in the number of erythrocytes by 26.5%, hemoglobin content by 8.7%, hemoglobin concentration in erythrocyte by 18.9%, increased weight of hemoglobin in erythrocyte by 24.1%, erythrocyte volume by 53.9%. In the application of the feed supplement «Butaselmevit Plus» to rats, under conditions of oxidative stress during research, the normalization of the activity of hematological parameters occurs in the blood, namely on the 25th and 30th days within the limits of physiological values were indicators of the number of erythrocytes, hemoglobin content, the number of leukocytes and indices of red blood compared with the control, indicating the restoration of hematopoietic function of the bone marrow.
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