• O. M. Melnichenko Institute of Veterinary Medicine of NAAS
  • A. Yu. Yushchenko State Scientific Control Institute of Biotechnology and Strains of Microorganisms
  • Z. S. Klestova State Scientific Control Institute of Biotechnology and Strains of Microorganisms
  • O. M. Deryabin State Scientific Control Institute of Biotechnology and Strains of Microorganisms
  • O. S. Vatlitsova State Scientific Control Institute of Biotechnology and Strains of Microorganisms
  • A. M. Golovko State Scientific Control Institute of Biotechnology and Strains of Microorganisms


Enterovirus infections remain one of the urgent problems in modern infectious pathology and are represented in numerous publications of domestic and foreign researchers, including publications in the field of veterinary virology. The causative agents of enterovirus infections of viral etiology (enteroviruses) are characterized by relative resistance to adverse environmental conditions, including thermal stability, acid resistance, resistance to proteolytic enzymes, which allows them to survive in the environment and facilitates their transmission by various ecological routes (water, food, aerosols, contaminated objects, etc.). The purpose of this study was to elucidate the changes in the infectious properties of porcine enteroviruses in vitro under conditions of long-term storage at a temperature of minus 32 °C. In the course of this study, a re- cultivation process was carried out with the subsequent adaptation of two variants of viruses: the porcine teschovirus of the first serotype (Teschovirus A), the “Dniprovsky 34” strain and Porcine sapelovirus 1 (porcine enterovirus of serogroup 8), the reference V-13 strain. The re-cultivation was performed on BHK-21 cell cultures / clone 13 and on SPEV, in which they were previously cultivated, in order to determine the infectious activity after storage under negative temperatures (minus 32 °C) for two and twenty years. On the example of porcine enterovirus of serogroup 8 (the causative agent of viral gastroenteritis), it was proved that during long-term storage (20 years) at a temperature of – 32 °C, the virus did not lose its infectious properties, although a change in the cytopathogenic effect in vitro during re-cultivation was found. The infectious properties of the porcine teshovirus of the first serotype are also capable of long-term storage (2 years) under conditions of minus 32 °C temperature.


Aimee, M., Gall Benito, J. Mariñas, Yi Lu, Joanna L. Shisler (2015). Waterborne Viruses: A Barrier to Safe Drinking Water, Published: June 25, 2015.

Andriushkova, N.H., Turchyna, N.S., Dolinchuk, L.V., Poniatovskyi, V.A., Melnyk, V.V., Shyrobokov, V.P., Trepet, L.M. (2016). Genome of enteroviruses in the serum of patients with acute cerebrovascular disorders. Medychni perspektyvy, 21, 4, 33-38. [in Ukrainian].

Babkin, M.V. (2006). Structural analysis of infectious diseases of pigs in Ukraine in modern conditions. Veterinarna meditsina: Mizhvid.tem.nauk.zbirnyk, 86, 25-30. [in Ukrainian].

Bova, T.O., Derevianko, S.V., Soroka, V.I., Bokun, A.O. (2004). Methodological approaches to creating a test system for the detection of antibodies to Teschen disease virus in pigs based on TIF. Naukovyi visnyk Lvivskoi derzhavnoi akademii veterynarnoi medytsyny imeni S.Z. Hzhytskoho, 6, 2, 3-9. [in Ukrainian].

Bova, T.O., Derevianko, S.V., Soroka, V.I. (2005). Optimization of solid-phase enzyme- linked immunosorbent assay for the indication of porcine teshoviruses Silskohospodarska mikrobiolohiia: mizhvid. temat. nauk. Zbirnyk, 1-2, 156-163. [in Ukrainian].

Bova, T.O., Derevianko, S.V., Soroka, V.I. (2005). Accelerated method of diagnosis of enzootic encephalomyelitis (Teschen disease) of pigs. Mikrobiolohichnyi zhurnal, 67, 6, 49-56. [in Ukrainian].

Bottner, A, Daneschnejad, S., Handrick, W. et al. (2002). A season of aseptic meningitis in Germany: epidemiologic, clinical and diagnostic aspects . Pediatr. Infect. Dis., 21, 12, 1126-1132.

Dayaram, A., Franz, M., Schattschneider, A., Damiani Armando, M., Bischofberger, S., Osterrieder, N. & Greenwood, Alex D. (2017). Long term stability and infectivity of herpesviruses in water, 1-10.

Demina, A.V., Shteynberg, A.V. (2009). Enteroviruses. Part 2. Enterovirus infections: variety of clinical manifestations. Byulleten SO RAMN, 6 (140), 116–125 [in Russian].

Doan, S. I. (2010). Enterovirus infections. Kyiv.: DIA, 9–28 [in Ukrainian].

Doan, S. I. (2006). Epidemiological analysis of the incidence of enterovirus infections, taking into account clinical manifestations, age and territorial distribution. Zaporozhskyi medytsynskyi zhurnal, 2, 62–67 [in Ukrainian].

Fauquet, C.M. (2005). Viruses Taxonomy: Eighth Report of the International Committee for the Taxonomy of Viruses New-York, San Diego: Academic Press, 657 – 673.

Fong, T.T., Lipp, E.K. (2005). Enteric viruses of humans and animals in aquatic environments: health risks, detection, and potential water quality assessment tools. Microbiol Mol Biol Rev., 69, 357–371. doi: 10.1128/MMBR.69.2.357-371.2005.

Hall, A.J. (2012). Noroviruses: the perfect human pathogens? J Infect Dis 205: 1622–1624. pmid:22573872.

International Committee on Taxonomy of Viruses Master Species List (2019),

Ischanova, I.Zh, Sagyinbaeva, B.A., Umarova, R.A. (2006). Modern clinical course of serous meningitis of enteroviral etiology. Vestnik Yuzhno-Kazahstanskoy Meditsinskoy Akademii, 2 (28), 58-59 [in Russian].

Kahn, M. (2010). Тhе Мегck Veterinary Manual. Tenth Edition. Published by Merck &CO. inc. Whitenouse Station, N.J., U.S.A., 2945.

Kehle, J., Roth, B., Metzger, C. et al. (2003). Molecular characterization of an Enterovirus 71 causing neurological disease in Germany. J. Neurovirol, 9, 1, 126-128.

Khetsuriani, N., La Monte-Fowlkes, A., M. Oberste, S., Pallansch Mark, A. (2006). Enterovirus Surveillance -- United States, 1970-2005, Surveillance Summaries, 1-20.

Klestova, Z.S. & Tatska, V.N. (2002). Ecological aspects of infectious mutagenesis of enterovirus etiology in swine genome pathology Veterynarna medytsyna: Mizhvidomchyi tematychny naukovyi zbirnyk, 82, 293 – 295. [in Ukrainian].

Klestova, Z.S. & Tatska, V.N. (2003). The effect of enteroviruses on the genetic apparatus of porcine cells. Animal biology, 5, 1-2, 257- 263. [in Ukrainian].

Martyinova, G.P. (2014). Enterovirus (non-polio) infection in children. Sibirskoe meditsinskoe obozrenie, 3,100–105 [in Russian].

Melnychenko, O.M., Mushtuk, I.Iu., Marchenko, V., Tashuta, S., Dremukh, Yu.. Klestova.

Z.S. (2014). Enteroviruses and their role in infectious pathology. Veterynarna biotekhnolohiia. 24, 105-114. [in Ukrainian].

Pallansch, M.A., Roos, R.P. (2001). Enteroviruses, polioviruses, coxsackieviruses and newer enteroviruses. Field Virology, 723-775.

Romanenko, V.P. (1974). Enterovirus diseases of pigs and their diagnosis. Veterynariia, 39, 53 – 55 [in Ukrainian].

Romanenko, V.F. (1984). Infectious gastrointestinal diseases of pigs: dovidnyk, 65–70 [in Russian].

Romanenko, V.F., Soroka, V.Y. (1995). Features of the epizootology of enzootic encephalomyelitis (Teschen disease) of pigs. General epizootology: immunological, ecological and methodological problems: сollection of scientific works, 92-95 [in Ukrainian].

Romanenko, V.P., Biloshtan, V.B., Sokolianskyi, I.F. (2007). Controllability of the infectious process in swine enterovirus diseases, 7, 34–36 [in Ukrainian].

Rotbart, H.A. (1995). Meningitis and encephalitis. Human Enterovirus Infections. Washington: ASM Press, 271-289.

Saeed, M., Zaidi, S., Naeem, A. et al. (2007). Epidemiology and clinical findings associated with enteroviral acute flaccid paralysis in Pakistan. BMC Infect.Dis.,7. doi: 10.1186/1471-2334-7-6.

Seidel, K.M., Goyal, S. M. Reo, V.Ch., Melnick, J.T. (1983). Concentration of rotavirus and enterovirus from blue crabs (Callinectes sapidus). Appl .and Environ. Microbiol, 6, 1283-1286.

Sereika, V. (2007). Seroprevalence of Antibudies against porcine teschovirus 1 in Lithuania. Acta Vet.Brno, 76, 231-236. DOI: 10.2754/avb200776020231.

Sereika, V., Lelesius, R., Zienius, D. (2007). Seroprevalence of Antibodies against porcine teschovirus 1 in Lithuania. Acta Vet. Brno, 76, 231-236.

Sergeev, V.A. (1993). Viral vaccines, 315 – 316. [in Ukrainian].

Shevtsova, N.P. & Golubeva M.V. (2004). Neurological manifestations of enterovirus infection. Children's infections, 3, 49-52. [in Ukrainian].

Shirobokov, V.P. (1974). The use of bentonite for the concentration of enteroviruses. Vaktsinyi i syivorotki., 8, 27-33. [in Russian].

Sorokina, M.N. & Skripnichenko, N.V. (2004). Viral Encephalitis and Meningitis in Children: A Guide for Physicians. [in Ukrainian].

Starcheus, A.P. (1987). Sensitivity of cell cultures of various origins to porcine enteroviruses. Increasing the efficiency of veterinary support for industrial pig breeding: mater. nauchno-prakt. respubl. konf., 78 –79. [in Russian].

Starcheus, A.P., Bondarenko, V.Y., Blotskaia, O.F., Zadorozhnaia, V.Y., Popovych, H.H., Vabyshchevych, F.S. (1988). Study of the antigenic relationship of human and animal intestinal enteroviruses. Kishechnyie infektsii: Respubl. mezhvedomstv. Sbornik, 20, 25-27 [in Ukrainian].

Syurin, V.N. (1998).Virusnyie bolezni zhivotnyih. Enterovirusnaya infektsiya sviney – M.: VNITIBP, 501 – 509. [in Russian].

Tatska, V.N. (2004). Diseases of pigs with viral gastroenteritis in modern conditions. Veterynarna biotekhnolohiia: biul., 5, 143 –152. [in Ukrainian].

UNICEF (2012) Pneumonia and diarrhoea: Tackling the deadliest diseases for the world's poorest children. New York, NY: Statistics and Monitoring Section- Division of Policy and Strategy.

Vorobeva, A.A. & Byikova, A.S. (2003). Atlas po meditsinskoy mikrobiologii, virusologii i immunologii. Moskva, Meditsinskoe inform. Agenstvo. 117 – 120 [in Russian].

Voroshilova, M.K. (1975). Enterovirusnyie infektsii cheloveka. M.: Meditsina: dovіdnyk. [in Russian].

Xu, W., Liu, CF., Yan, L. et al. (2012). Distribution of enteroviruses in hospitalized children with hand, foot and mouth disease and relationship between pathogens and nervous system complications,Virol J., 9, 8.

Yamada, M., Kozakura, R., Ikegami, R., Nakamura, K., Kaku, Y., Yoshii, M. & Haritani, M. (2004). Enterovirus encephalomyelitis in pigs in Japan caused by porcine teschovirus. Vet. Rec., 155, 304–306 in Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, 8th Edition (2018), 1- 3, 1618-1619. doi: 10.1136/vr.155.10.304.

Zell, R. (2001). Porcine teschovirus comprise at least eleven distinct serotypes: molecular and evolutionary aspects. J. Virol., 75,4, 1620 – 1631. doi: 10.1128/JVI.75.4.1620-1631.2001.

How to Cite
Melnichenko, O. M., Yushchenko, A. Y., Klestova, Z. S., Deryabin, O. M., Vatlitsova, O. S., & Golovko, A. M. (2020). THE CULTURAL PROPERTIES ALTERATIONS OF PORCINE ENTEROVIRUS DURING LONG-TERM STORAGE. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 21(2), 123-136.