SURFACE PLASMON RESONANCE METHOD FOR DETECTION CHICKEN INFECTIOUS BRONCHITIS CORONAVIRUS

  • Z. S. Klestova State Scientific Control Institute of Biotechnology and Strains of Microorganisms State Service of Ukraine on Food Safety and Consumer Protection
  • A. K. Voronina State Scientific Control Institute of Biotechnology and Strains of Microorganisms State Service of Ukraine on Food Safety and Consumer Protection
  • A. Yu. Yushchenko State Scientific Control Institute of Biotechnology and Strains of Microorganisms State Service of Ukraine on Food Safety and Consumer Protection
  • O. S. Vatlitsova State Scientific Control Institute of Biotechnology and Strains of Microorganisms State Service of Ukraine on Food Safety and Consumer Protection
  • G. V. Dorozinsky V.E. Lashkaryov Institute of Semiconductor Physics NAS of Ukraine National Academy of Sciences of Ukraine
  • Yu. V. Ushenin V.E. Lashkaryov Institute of Semiconductor Physics NAS of Ukraine National Academy of Sciences of Ukraine
  • V. P. Maslov V.E. Lashkaryov Institute of Semiconductor Physics NAS of Ukraine National Academy of Sciences of Ukraine
  • T. P. Doroshenko V.E. Lashkaryov Institute of Semiconductor Physics NAS of Ukraine National Academy of Sciences of Ukraine
  • S. O. Kravchenko V.E. Lashkaryov Institute of Semiconductor Physics NAS of Ukraine National Academy of Sciences of Ukraine
Keywords: SURFACE PLASMON RESONANCE (PPR), CORONVIRUSES, DETECTION.

Abstract

The article presents a new developed method, which is able to detect the chicken infectious bronchitis virus (IBV) antigen in real time in various buffer solutions, using the surface plasmon resonance (PPR) nanobiosensor of the Plasmon-6 device. The PPR method is hypersensitive to changes in external factors, including the interaction of antigen (coronavirus) and specific antibodies. If the interaction does not happen, the resonance occurs at other angular parameters of the position of the sensitive PPR element relative to the laser radiation. Therefore, the PPR method is becoming a new effective rapid technique of viral pathogen detection, which is important for effective control over infectious diseases spreading. The possibility of IBK virus detection by the PPR sensor response, with preliminary immobilization of antigen or antibodies, is shown, involving the device "Plasmon- 6". The duration of the experiment is about 2 hours, which significantly saves research time compared to other methods (6-48 hours). The changes in the resonance angle in the range of 360-500 angle. sec when the IBC virus antigen binds to serum antibodies in water (distilled) were detected. The angular shift of the nanosensor resonance was determined when the IBC virus antigen bound to the serum antibodies in the PBS, which averaged 354 angular seconds. The possibilities of using the PPR method for express detection of the coronavirus infections pathogen in animal fluids in real time are demonstrated in article. Taking into account the significant social and economic negative consequences of the Coronaviridae virus family members and considering the current situation with the worldwide spread of COVID-19, the representative of the coronavirus family – the Infectious Bronchitis virus has been selected as a model.

References

Akan, M., Izgur, M., Sareyyupolu, B. (2007). Diagnosis of infectious bronchitis in chickens by polymerase chain reaction and fluorescent antibody technique. Veteriner Fakültesi dergisi, 54(1), 47-54.

Brockman, G., Nelson, P., Corn, R. (2000). Surface plasmon resonance imaging measurements of ultrathin organic films. Annu. Rev. Phys. Chem., 51, 41-63. https://doi.org/10.1146/annurev.physchem.51.1.41

Cavanagh, D. (2003). Severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus. Avian Pathol., 32, 567–582. https://doi.org/10.1080/03079450310001621198

Cavanagh, D. & Gelb, J. (2008). Infectious Bronchitis. In: Saif, Y.M. (Ed.), Diseases of Poultry 12th Ed. Blackwell Publishing Ltd., Ames, Iowa, 117–135.

Chen, Y., Jiang, L, Zhao, W., Liu, L., Zhao, Y., Shao, Y., Li, H., Han, Z., Liu, S. (2017). Identification and molecular characterization of a novel serotype infectious bronchitis virus (GI‐28) in China. Veterinary Microbiol, 198, 108–115. https://doi.org/10.1016/j.vetmic.2016.12.017

Ionov, I.A., Tereshenko, O.V., Katerinich, O.O. (2012). Promising program «Development of the poultry industry until 2020», Efektivne ptakhivnicztvo, 10, 12-22. [in Ukrainian].

Ha-Jung, R., Hilt, D., Jackwood, M. (2014). Detection of infectious bronchitis virus with the use of real-time quantitative reverse transcriptase-PCR and correlation with virus detection in embryonated eggs. Avian Dis., 58, 398–403. https://doi.org/10.1637/10764-010914-Reg.1

Khan, M., Hasan, M., Hossain, S., Ahommed, M., Daizy, M. (2020). Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: state of the art. Biosens. Bioelectron, 166, 112431–112444. https://doi.org/10.1016/j.bios.2020.112431

https://doi.org/10.20535/ibb.2019.3.1.163106

Liu I., Lin Y., Jian C., Cheng I., Chen H. (2019). A novel immunochromatographic strip for antigen detection of avian infectious bronchitis virus. Int J. Mol. Sci., 20, 2216– 2226. https://doi.org/10.3390/ijms20092216

Valastro, V., Holmes, E., Britton, P., Fusaro, A., Jackwood, M., Cattoli, G., Monne, I. (2016). S1 gene-based phylogeny of infectious bronchitis virus: an attempt to harmonize virus classification. Infect Genet Evol., 39, 349–364. https://doi.org/10.1016/j.meegid.2016.02.015

Van Beurden, S., Berends, A., Kramer-Kuhl, A., Spekreijse, D., Chenard, G., Philipp, H., Mundt, E., Rottier, P., Verheije, M. (2018). Recombinant live attenuated avian coronavirus vaccines with deletions in the accessory genes 3ab and/or 5ab protect against infectious bronchitis in chickens. Vaccine, 36, 1085–1092. https://doi.org/10.1016/j.vaccine.2018.01.017

Venger, E., Maslov, V., Ushenin, Yu., Kravchenko S., Dorozhynskyi G., Holovko, A., Klestova, Z., Blotskaya. O., Yushchenko, A. (2020). Sposob izgotovleniya chuvstvitelnogo elementa immunosensora na osnove yavleniya poverkhnostnogo plazmonnogo rezonansa dlya diagnostiki lejkoza krupnogo pogatogo skota. Mezhdunarodnyj petent WO 2020/018060 A1 2020. [in Ukrainian].

Published
2020-10-27
How to Cite
Klestova, Z. S., Voronina, A. K., Yushchenko, A. Y., Vatlitsova, O. S., Dorozinsky, G. V., Ushenin, Y. V., Maslov, V. P., Doroshenko, T. P., & Kravchenko, S. O. (2020). SURFACE PLASMON RESONANCE METHOD FOR DETECTION CHICKEN INFECTIOUS BRONCHITIS CORONAVIRUS. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 21(2), 48-56. https://doi.org/10.36359/scivp.2020-21-2.06