ACTIVITY AND CONTENT OF SUPEROXIDEDISMUTASE IZOZYMES IN GRANULOSE CELLS FROM COW OVARY FOLLICLES

  • Yu. V. Bodnar Institute of Animal Biology of NAAS (Lviv, Ukraine)
  • N. V. Kuzmina Institute of Animal Biology NAAS (Lviv, Ukraine)
  • D. D. Ostapiv Institute of Animal Biology NAAS (Lviv, Ukraine)
  • S. W. Kawa Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies
  • O. I. Chajkovska State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives
  • R. D. Ostapiv State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives
Keywords: SUPEROXIDE DISMUTASE, GRANULOSE CELLS, FOLLICLES, OVARIES, COWS.

Abstract

The activity and content of superoxide dismutase isoforms (SOD) in granulosa cells from cow ovarian follicles were studied for research after slaughter of cows ovaries were selected, which according to the physiological state were divided into groups: with "fresh" ovulation, at the site of the ovulated follicle there is a hole, no corpus luteum or diameter up to 5 mm, red color (n = 14); with "early corpus luteum", diameter 10-20 mm, color red or brown (n = 41); with “late corpus luteum", diameter 5–15 mm, color yellow (n = 32); "follicular growth", without the corpus luteum (n = 84). The ovaries of cows with small (<4 mm), medium (4 - 7 mm) and large (> 7 mm) follicles were used. Antral fluid was obtained from the follicles, from which granulosa cells were isolated. Cells were suspended according to the volume of follicular fluid in the medium Dulbeccos modified Eagle medium (DME) with the addition of estrus serum of cows, follicular fluid, insulin and heparin. In cell culture, protein concentration, activity, and superoxide dismutase isozymes were determined.

It was found that granulosa cells are characterized by SOD activity - 12.4 ± 0.74 IU / mg protein (6.8 ± 1.72 - 19.8 ± 3.75 IU / mg protein). The activity of SOD in the culture of granulosa cells had 5–6 isoforms of the enzyme. It was found that isoforms at the site of localization are divided into cytosolic, mitochondrial and extracellular proteins of SOD. The cytosolic isoform were represented by 3 - 4, and mitochondrial and extracellular have one active protein of the enzyme. he activity of the enzyme and the content of isoforms depended on the size of the follicles from which the cells are removed and the physiological state of the ovaries. The studied indicators characterize the intensity of oxidative metabolism as a whole in cells and in their individual parts and organelles. For cultivation, it is advisable to select granulosa cells from ovarian follicles of "follicular growth" and "late corpus luteum" because they are characterized by consistently high activity of SOD, which protects intracellular components from the cytotoxic action of superoxide anion.

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Published
2020-10-27
How to Cite
Bodnar, Y. V., Kuzmina, N. V., Ostapiv, D. D., Kawa, S. W., Chajkovska, O. I., & Ostapiv, R. D. (2020). ACTIVITY AND CONTENT OF SUPEROXIDEDISMUTASE IZOZYMES IN GRANULOSE CELLS FROM COW OVARY FOLLICLES. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 21(2), 33-39. https://doi.org/10.36359/scivp.2020-21-2.04