The influence of polyhexamethylene guanidine salts on the general development and status of the central nervous system of rats

  • I. M. Kushnir State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
  • G. V. Kolodiy State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
  • V. I. Kushnir State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
  • I. S. Semen State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
  • S. D. Murska State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
Keywords: polyhexamethyleneguanidine, rats, central nervous system, behavior reactions, open field, movement activity

Abstract

It was conducted the determination of the influence of disinfectant, which is a 25% aqueous solution of salts of polyhexamethyleneguanidine hydrochloride and polyhexamethylene guanidine -phosphate, on the emotional and motor activity of rats. The research was made according to the generally accepted methodology in open field test. The assesment of the influence of the investigated mean on the behavior of white rats of the generations F1 and F2 was carried out by comparison with the corresponding results of the control and three experimental groups.

The results of the experiment indicate on the influence of the investigated mean on the central nervous system of animals in the experimental group IV, in generations of F1 and F2, which got a drug in a concentration of 1.2 mg/kg body weight. It was found out the decrease such indicators as the amount of vertical racks on the hind legs, the amount of looking into the holes and the amount of washings. Herewith the amount of acts of defecation and urination in animals of the IV experimental group was increased, which may indicate into the development of inhibition of the central nervous system and the emergence of an emotional reaction to fear. As a result of the studies, it was found out that the use of the investigated mean, created on the basis of polyhexamethylene guanidine salts, in the second experimental group did not change the emotional and motor activity of animals.

In determining the indicators of postnatal development of F1 and F2 generation of rats, it was found out that the appearance of fur, eye opening, and peeling off the auricle in control animals and the second experimental group appeared at the same time. Whereas in the 3rd and 4th experimental groups, which got the investigated means  in a concentrations, respectively, 0.6 and 1.2 mg/kg body weight, noted that fur covering happened over 7-8 days after birth, eye opening, respectively, on   16 and 17 days. Furthermore, peeling off the auricle  in the animals of the 4th experimental group, it also occurred later, in particular, on the 7 days.

References

Zhou Z. Interactions of biocidal guanidine hydrochloride polymer analogs with model membranes: a comparative biophysical study / Z. Zhou, A. Zheng, J. Zhong // Acta Biochimica et Biophysica Sinica. – 2011. – Vol. 43. – № 9. – P. 729–737.

Artemova T. Z. The problem in the reactivation of microorganisms on evaluating the efficacy of water disinfectants / T. Z. Artemova, A. E. Nedachin, Z. I. Zholdakova [еt al] // Gig. Sanit. – 2010. – Vol. 1. – Р. 15–18.

Yakubchak О. M. Veterinarna dezinfekcia. К.: Коmpania Bioprom . – 2010. – 152 s. (in Ukrainian).

European convention for the protection of vertebrate animals used for experimental and other scientifi c purposes // Council of European. – Strasbourg. – 1986. – №123. – 51 р.

Suchasni problemy bioetyky / Vidp. red. Y. I. Kundiev. – К.: «Academiperiodyca». – 2009. – 278 s. (in Ukrainian).

Buresh Y. Methodiky i osnovnye experimenty po izycheniy mozga y povedenia / Y. Buresh, O. Bureshova, D. Houston // M .: Visch. shcola. - 1991 - 399 s. (in Russian).

Hall C. S. A study of the rat's behavior in a field: a contribution to method in comparative psychology / C. S. Hall, E. L. Ballachey // University of California Publications in Psychology. – 1932. – Vol. 6. – P. 1–12.

Кaluev А. V. Stress, trevognost i povedenie: actyalnye problemy modelirovania trevognogo povedenya u gyvotnykh / А. V. Кaluev // – К. : CSF. – 1998. – 98 s. (in Russian).

Published
2019-09-18
How to Cite
Kushnir, I. M., Kolodiy, G. V., Kushnir, V. I., Semen, I. S., & Murska, S. D. (2019). The influence of polyhexamethylene guanidine salts on the general development and status of the central nervous system of rats. Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology, 20(2), 81-87. https://doi.org/10.36359/scivp.2019-20-2.11